On detecting reference level of acrolein content in children's blood

View or download the full article: 
UDC: 
614.71
Authors: 

T.S. Ulanova1,3, O.O. Sinitsyna2, T.D. Karnazhitskaya1, E.O. Zavernenkova1

Organization: 

1 Federal Scientific Center for Medical and Preventing Health Risk Management Technologies, 82 Monastyrskaya Str., Perm, 614045, Russian Federation
2 Scientific Research Institute for Human Ecology and Environment Hygiene named afetr А.N. Syisin, 10, build. 1, Pogodinskaya Str., Moscow, 119992, Russian Federation
3 Perm National Research Polytechnic University, 29 Komsomolskiy avenue, Perm, 614990, Russian Federation

Abstract: 

The article gives the results of complex chemical-analytical and clinical-laboratory research in course of which biological media of children living in Perm region were examined. To study impacts exerted by exogenous acrolein we examined 156 children in 2014–2016, aged 5–10, attending pre-school facilities and schools, and living in Perm region.

As we conducted this research we detected average annual acrolein concentration in atmosphere on the examined territory; this concentration was equal to 0.000024 mg/m3, and it was 1.2 times higher than reference acrolein concentration in the air for chronic inhalation exposure. Average group acrolein concentration in children's blood was 1.2 times authentically higher (р<0.05) than regional background level of acrolein content in blood of children living on conditionally clean (control) territory of Perm region. Average content of malonic dialdehyde in blood plasma and IgG specific to acrolein was 1.2 and 1.4 times authentically higher than physiological standard for these parameters (р<0.05). Average group concentration of delta-aminolevulinic acid in urine was detected at the top limit of physiological standard.

Applying odds relation criterion (OR=e a0–a1x ) we obtained authentic models for correlation between acrolein content in blood and G immunoglobulin specific to acrolein, antioxidant activity of blood plasma, crude bilirubin in blood, and delta-aminolevulinic acid in urine (F>3.96, p≤0.05). We used increased content of delta-aminolevulinic acid in urine as a limiting marker for effects occurring at chronic inhalation exposure to acrolein. Basing on the results of the performed examination we recommend concentration equal to 0.10 mgr/dm 3 as a reference level of acrolein content in blood at chronic inhalation exposure.

Keywords: 
acrolein, chronic exposure, response markers, blood, highly efficient liquid chromatography, delta-aminolevulinic acid, bilirubin, reference level
Ulanova T.S., Sinitsyna O.O., Karnazhitskaya T.D., Zavernenkova E.O. On detecting reference level of ac-rolein content in children's blood. Health Risk Analysis, 2017, no. 1, pp. 29–37. DOI: 10.21668/health.risk/2017.1.04.eng
References: 
  1. Akrolein [Acrolein]. Nauchnye obzory sovetskoi literatury po toksichnosti i opasnosti khimicheskikh veshchestv [Scientific overviews of Soviet literature on chemical substances toxicity and danger]. In: N.F. Izmerov ed. Moscow, 1984, no. 50, 15 p. (in Russian).
  2. Vrednye veshchestva v promyshlennosti: spravochnik [Hazardous substances in industry: guidance]. In: N.V. Lazareva and E.II. Levinoi eds. Leningrad, Khimiya Publ., 1976, vol. I, 508 p. (in Russian).
  3. Drugov Yu.S., Rodin A.A. Monitoring organicheskikh zagryaznitelei prirodnoi sredy: prakticheskoe rukovodstvo [Environment organic pollutants monitoring: practical guidelines]. Moscow, BINOM. Laboratoriya znanii Publ., 2009, 893 p. (in Russian).
  4. Konovalova E.V. Zashchitnoe deistvie karnozina, vklyuchennogo v sostav nanoliposom, v usloviyakh okislitel'nogo stressa in vitro i in vivo: dis…kandid. biol.nauk [Protective effect of carnosine, included into nanoli-posome structure, under oxidative stress in vitro and in vivo: abstract of a thesis…candidate of biological sciences]. Moscow, 2013, 165 p. (in Russian).
  5. Laboratornye metody issledovaniya v klinike: spravochnik [Laboratory research techniques in a clinic: guide]. In: V.V. Men'shikov ed. Moscow, Meditsina Publ., 1987, 366 p. (in Russian).
  6. MR 2.1.10.0062-12. Kolichestvennaya otsenka nekantserogennogo riska pri vozdeistvii khimicheskikh veshchestv na osnove postroeniya evolyutsionnykh modelei: metodicheskie rekomendatsii 2.1.10.0062-12 [Quantitative assessment of non-carcinogenic risk under exposure to chemicals based on evolutionary models design: methodical guidelines 2.1.10.0062-12]. Kodeks: elektronnyi fond, 2012. Available at: http://docs.cntd.ru/document/1200095225 (11.10.2016) (in Russian).
  7. MUK 4.1.3158–14. Izmerenie massovoi kontsentratsii akroleina v krovi metodom vysokoeffektivnoi zhidkostnoi khromatografii: metodicheskie ukazaniya [Measuring mass acrolein concentration in blood using highly efficient liquid chromatography: methodical guidelines]. Kodeks: elektronnyi fond, 2014. Available at: http://docs.cntd.ru/document/1200119565 (11.10.2016) (in Russian).
  8. MUK 4.1.3356-16. Izmerenie massovoi kontsentratsii akroleina v atmosfernom vozdukhe metodom vysokoeffektivnoi zhidkostnoi khromatografii: metodicheskie ukazaniya [Measuring mass acrolein concentration in atmosphere using highly efficient liquid chromatography: methodical guidelines]. Kodeks: elektronnyi fond, 2016. Available at: http://docs.cntd.ru/document/456022815 (11.10.2016) (in Russian).
  9. Zavernenkova E.O., Ulanova T.S., Sinitsyna O.O., Karnazhitskaya T.D. Obosnovanie regional'nykh fonovykh urovnei soderzhaniya akroleina v biosredakh (krov', mocha) naseleniya na primere Permskogo kraya [Substantiating regional background levels of acrolein content in biological media (blood and urine) of population by the example of Perm region]. Okruzhayushchaya sreda i zdorov'e. Gigiena i ekologiya urbanizirovannykh territorii: materialy VI Vserossiiskoi nauchno-prakticheskoi konferentsii molodykh uchenykh i spetsialistov s mezhdunarodnym uchastiem [Environment and health. Hygiene and ecology of urbanized areas: materials of VI Russian theoretical and practical conference of young scientists and specialists with international participation]. 2016, pp. 210–215 (in Russian).
  10. Uait A., Khendaer F., Smit E., Khial R., Leman I. Osnovy biokhimii [Basics of biochemistry]. Moscow, Mir Publ., 1981, vol. 2, pp. 540–1152 (in Russian).
  11. Rukovodstvo po ocenke riska dlja zdorov'ja naselenija pri vozdejstvii himicheskih veshhestv, zagrjaznjajushhih okruzhajushhuju sredu R 2.1.10.1920-04 [Guide to health risk assessment when exposed to chemicals polluting the environment 2.1.10.1920-04 P]. Moscow: Federal'nyj centr Gossanjepidnadzora Minzdrava Rossii, 2004, 143 p. (in Russian).
  12. Filov V.A., Tiunov L.A. Vrednye khimicheskie veshchestva. Galogen- i kislorodsoderzhashchie organi-cheskie soedineniya: spravochnik [Hazardous chemicals. Halogen- and oxygen-containing organic compounds: guide]. St. Petersburg, Khimiya Publ., 1994, 286 p. (in Russian).
  13. Fletcher R., Fletcher S., Vagner E. Klinicheskaya epidemiologiya. Osnovy dokazatel'noi meditsiny [Clini-cal epidemiology. Basics of evidential medicine]. Moscow, Media Sfera Publ., 1998, 352 p. (in Russian).
  14. Khimicheskaya entsiklopediya [Chemical encyclopedia]. In: I.L. Knunyants ed. Moscow, Sovetskaya entsiklopediya Publ., 1988, vol. 1, 623 p. (in Russian).
  15. Chetyrkin E.M. Statisticheskie metody prognozirovaniya [Statistic prediction techniques]. Moscow, Sta-tistika, 1977, 356 p. (in Russian).
  16. Acrolein Reference Exposure Levels DRAFT. Draft TSD for Noncancer RELs, SRP4, 2008, 26 p. Availa-ble at: http://oehha.ca.gov/media/downloads/air/document/acrolein112508.pdf (18.10.2016).
  17. Finkelstein E., Nardini M., Vliet A. Inhibition of neutrophil apoptosis by acrolein: a mechanism of tobac-co-related lung disease? Am. J. Physiol. Lung. Cell. Mol. Physiol, 2001, vol. 281, no. 3, pр. 732–739.
  18. Sakata K., Kashiwagi K., Sharmin S., Ueda S., Irie Y., Murotani N., Igarashi K. Increase in putrerscine, amine oxidase, and acrolein in plasma of renal failure patients. Biochem Biophys Res Commun, 2003, vol. 305, no. 1, pp. 143–149.
  19. Li L., Holian A. Acrolein: a respiratory toxin that suppressespulmonary host defense. J. Environmental Health, 1998, no. 13, pp. 99–108.
  20. Lou J., Robinson P., Shi R. Acrolein-induced cell death in PC12 cells: role of mitochondria-mediated oxidative stress. Neurochemistry international, 2005, vol. 47, no. 7, pp. 449–457.
  21. Luo J., Shi R. Acrolein induces oxidative stress in brain mitochondria. Neurochemistry International, 2005, vol. 46, no. 3, pp. 243–252.
  22. Nazaroff W., Singer B. Inhalation of hazardous air pollutants from environmental tobacco smoke in US residences. J. Expo. Anal. Environ. Epidemiol, 2004, 14, Suppl 1, pp. 71–77.
  23. Toxicological Profile for Acrolein. U.S. Department of Health and Human Services. Health Service Agency for Toxic Substances and Disease Registry, 2007. Available at: https://www.atsdr.cdc.gov/toxprofiles/tp124.pdf (28.10.2016).

You are here