Adolescent smoking as risk factor of decrease in adaptation resources of neutrophil metabolism in oral cavity and progressive recurrent bronchitis

UDC: 
616.155.34:616.311+616.234-002-02:613.84-053.6
DOI: 
10.21668/health.risk/2024.3.13.eng
Authors: 

O.I. Pikuza1, A.M. Zakirova1, Kh.M. Vakhitov1, E.V. Volanyuk2, E.L. Rashitova3, A.A. Pikuza1

Organization: 

1Kazan State Medical University, 49 Butlerova St., Kazan, 420012, Russian Federation
2Kazan State Medical Academy – Branch Campus of the Federal State Budgetary Educational Institution of Further Professional Education «Russian Medical Academy of Continuous Professional Education», 11 Mushtari St., Kazan, 420012, Russian Federation
3Dmitry Rogachev National Medical Research Center of Pediatric Hematology, Oncology and Immunology, 1 Samory Mashela St., Moscow, 117198, Russian Federation

Abstract: 

High prevalence of smoking among children and its early onset is an urgent problem in modern pediatrics. For proper health risk quantification, it is extremely important to understand and parameterize relationships between effects of adverse factors and physiological processes in the body, including the cellular level.

The aim of this study was to analyze how tobacco smoking influences maladaptive changes in the mucosal immunity system of the oral cavity in adolescents and to assess kinetic trends in the clinical course of recurrent bronchitis (RB) in long-term outlook.
The study included 92 patients with RB aged 16.8 ± 3.1 years. Two groups were created of them; the observation group was made of 64 patients, who admitted smoking as a habit, and the reference group included 28 people without nicotine addiction. The control group was made of 23 adolescents of the same age without any signs of the analyzed disease.

Oral neutrophils (N) were selected as basic research material. N oxygen metabolism was registered by luminol-dependent chemiluminescence (LDCL) in spontaneous (sLDCL) and induced (iLDCL) variants. Functional N probing was performed using opsonized zymosan (Σ) and and peptidoglycan from S. aureus strain Cowan. Concentrations of antibodies (AT) to glycolipid (GLP) (Re-mutant Salmonella Minnesota), Candida albicans and S. аureus were determined by ELISA tests.

The analyses made it possible to detect a close relationship between levels of ROS generation by neutrophilic granulocytes and the immune components of anti-endotoxic immunity. Immune changes were not established to be universal in the groups of examined patients and were shown to reflect the specificity of destabilizing effects produced by tobacco smoke. A direct moderate correlation was established in the observation group between sLDCL indicators and the concentration of anti-candidosis antibodies (r = +0.59, p = 0.0382), which reflects the level of bacterial stimulation involving dysbiotic shifts in gut microbiota. A direct correlation was detected between levels of biocidal parameters of neutorphil granulocytes and anti-glycolipid levels (r = +0.64 p = 0.417).

Integral assessment of specific humoral immunity to glycolipid and the phagocytic link of the mucosal protection in the oral cavity reflects the degree of endogenous intoxication and kinetic trends in the RB clinical course in adolescents in subsequent years of life. These relationships may provide solid rounds for ‘exposure – response’ stage in the risk assessment procedure for assessing risks of respiratory diseases in smoking adolescents.

Keywords: 
adolescents, smoking, recurrent bronchitis, mucosal immunity, oral neutrophilic granulocytes, endotoxin aggression, anti-endotoxic immunity, prediction
Pikuza O.I., Zakirova A.M., Vakhitov Kh.M., Volanyuk E.V., Rashitova E.L., Pikuza A.A. Adolescent smoking as risk factor of decrease in adaptation resources of neutrophil metabolism in oral cavity and progressive recurrent bronchitis. Health Risk Analysis, 2024, no. 3, pp. 123–131. DOI: 10.21668/health.risk/2024.3.13.eng
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Received: 
05.06.2024
Approved: 
19.09.2024
Accepted for publication: 
23.09.2024

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