Mycotoxins in coffee and chicory: from regulated to emergent
I.B. Sedova, M.G. Kiseleva, Z.A. Chalyy
Federal Research Centre of Nutrition, Biotechnology and Food Safety, 2/14 Ustinskiy proezd, Moscow, 109240, Russian Federation
Coffee is a daily basic food product for many people all over the world. In Russia and some European countries, people who try to pursue healthy lifestyle often prefer chicory as a substitute to coffee. Our research goal was to evaluate occurrence of Aspergillus, Penicillium, Fusarium and Alternaria secondary metabolites in coffee and chicory distributed on the RF market.
29 mycotoxins were determined in 48 samples of coffee and chicory using ultra high-performance liquid chromatography coupled with tandem mass-spectrometric detection (UHPLC-MS/MS).
The range of analyzed contaminants included regulated mycotoxins (aflatoxins, ochratoxin A, deoxynivalenol, fumonisins, T-2 toxin, and zearalenone), their derivatives and structural analogs (A and B trichothecenes), Alternaria metabolites (alternariol, its methyl ether, altenuene, tentoxin), citrinin and several emergent mycotoxins (citreoviridin, cyclopiazonic and mycophenolic acids, enniatins, beauvericin).
To the best of our knowledge, the present study is the first to report results indicating that unregulated emergent mycotoxins occur in the examined products. Chicory samples contained beauvericin (9 of 16 samples, the contents varied from 2.4 to 1173 µg/kg) and enniatin B (6 of 16 samples, 2.8–1109 µg/kg). Green and roasted coffee samples contained mycophenolic acid (11 of 20 samples, 23.5–58.3 µg/kg; 3 of 12 samples, 155.7–712.2 µg/kg accordingly). Several samples were contaminated with aflatoxins, ochratoxin A and fumonisin B2. Their contents in the examined samples did not exceed maximum levels; however, their occurrence indicates a potential health risk for consumers. This requires hygienic assessment and monitoring of these products with the focus on their contamination not only with regulated aflatoxin B1 and ochratoxin A but also with other potentially hazardous mycotoxins.
- Toci A.T., Farah A., Pezza H.R., Pezza L. Coffee Adulteration: More than Two Decades of Research. Crit. Rev. Anal. Chem., 2016, vol. 46, no. 2, pp. 83−92. DOI: 10.1080/10408347.2014.966185
- Ergin E., Tokusoglu O., Vural H. Coffee toxicology, processing of the coffee and liver
diseases (is it a miracle of nature?). Food Process. Preserv., 2021, vol. 45, no. 4, pp. e15243. DOI: 10.1111/jfpp.15243 - Vidy i sorta kofe [Types and varieties of coffee]. Available at: https://bengusta.com.ua/blog/
likbez/vidy-i-sorta-kofe/ (21.11.2021) (in Russian). - Ludwig I.A., Clifford M.N., Lean M.E.J., Ashihara H., Crozier A. Coffee: biochemistry and potential impact on health. Food Funct., 2014, vol. 5, no. 8, pp. 1695–1717. DOI: 10.1039/c4fo00042k
- Vierra V., Cunha S., Casal S. Mycotoxins in coffee. Coffee in Health and Disease Prevention, 2015, Chapter 25, pp. 225–233. DOI: 10.1016/B978-0-12-409517-5.00025.5
- Ramirez L.M., Cendoya E., Nichea M.J., Zachetti V.G.L., Chulze, S.N. Impact of toxigenic fungi and mycotoxins in chickpea: a review. Current Opinion in Food Science, 2018, vol. 23, pp. 32–37. DOI: 10.1016/j.cofs.2018.05.003
- Abdel-Hadi A., Magan N. Influence of physiological factors on growth, sporulation and ochratoxin A/B production of the new Aspergillus ochraceus grouping. World Mycotoxin J., 2009, vol. 2, no. 4, pp. 429–434. DOI: 10.3920/WMJ2009.1156
- Benites A.J., Fernandes M., Boleto A.R., Azevedo S., Silva S., Leitao A.L. Occurrence of ochratoxin A in roasted coffee samples commercialized in Portugal. Food Control, 2017, vol. 73, part B, pp. 1223–1228. DOI: 10.1016/j.foodcont.2016.10.037
- Perez De Obanos A., Gonzalez-Penas E., Lopez De Cerain A. Influence of roasting and brew preparation on the ochratoxin A content in coffee infusion. Food Additives and Contaminants, 2005, vol. 22, no. 5, pp. 463–471. DOI: 10.1080/02652030500090042
- Bessaire T., Perrin I., Tarres A., Bebius A., Reding F., Theurillat V. Mycotoxins in green coffee: Occurrence and risk assessment. Food Control, 2019, vol. 96, pp. 59–67. DOI: 10.1016/j.foodcont.2018.08.033
- Lindenmeier M., Schieberle P., Rychlik M. Determination of ochratoxin A in food: comparison of a stable isotope dilution assay, liquid chromatography-fluorescence detection and an enzyme-linked immunosorbent assay. Mycotoxin Res., 2011, vol. 27, no. 2, pp. 115–121. DOI: 10.1007/s12550-010-0084-1
- Lee T.P., Saad B., Khayoon W.S., Salleh B. Molecularly imprinted polymer as sorbent in micro-solid phase extraction of ochratoxin A in coffee, grape juice and urine. Talanta, 2012, vol. 88, pp. 129–135. DOI: 10.1016/j.talanta.2011.10.021
- Khaneghah A.M., Fakhri Y., Abdi L., Coppa C.F.S.C., Franco L.T., Fernandes de Oliveira C.A. The concentration and prevalence of ochratoxin A in coffee and coffee-based products: A global systematic review, meta-analysis and meta-regression. Fungal Biol., 2019, vol. 123, no. 8, pp. 611–617. DOI: 10.1016/j.funbio.2019.05.012
- Culliao A.G.L., Barcelo J.M. Fungal and mycotoxin contamination of coffee beans in Benguet province, Philippines. Food Addit. Contam. Part A Chem. Anal. Control Expo. Risk Assess., 2015, vol. 32, no. 2, pp. 250–260. DOI: 10.1080/19440049.2014.1001796
- Barcelo J.M., Barcelo R.C. Post-harvest practices linked with ochratoxin A contamination of coffee in three provinces of Cordillera Administrative Region, Philippines. Food Addit. Contam. Part A Chem. Anal. Control Expo. Risk Assess., 2018, vol. 35, no. 2, pp. 328–340. DOI: 10.1080/19440049.2017.1393109
- Ayelign A., De Saeger S. Mycotoxins in Ethiopia: Current status, implications to food safety and mitigation strategies. Food Control, 2020, vol. 113, pp. 107163. DOI: 10.1016/j.foodcont.2020.107163
- Nielsen K.F., Ngemela A.F., Jensen L.B., de Medeiros L.S., Rasmussen P.H. UHPLC-MS/MS determination of ochratoxin A and fumonisins in coffee using QuEChERS extraction combined
with mixed-mode SPE purification. J. Agric. Food Chem., 2015, vol. 63, no. 3, pp. 1029–1034. DOI: 10.1021/jf504254q - Casal S., Vieira T., Cruz R., Cunha S.C. Ochratoxin A in commercial soluble coffee and coffee substitutes. Food Res. Int., 2014, vol. 61, pp. 56–60. DOI: 10.1016/j.foodres.2014.04.045
- García-Moraleja A., Font G., Mañes J., Ferrer E. Analysis of mycotoxins in coffee and risk assessment in Spanish adolescents and adults. Food Chem. Toxicol., 2015, vol. 86, pp. 225–233. DOI: 10.1016/j.fct.2015.10.014
- Karlovsky P., Suman M., Berthiller F., De Meester J., Eisenbrand G., Perrin I., Oswald I.P., Speijers G. [et al.]. Impact of food processing and detoxification treatments on mycotoxin contamination. Mycotoxin Research, 2016, vol. 32, no. 4, pp. 179–205.
- Sedova I.B., Kiseleva M.G., Zakharova L.P., Tutelyan V.A. Toxicological and hygienic characteristics of mycotoxin sterigmatocystin and methods for its determination in food products. Gigiena i sanitariya, 2019, vol. 98, no. 1, pp. 105–117. DOI: 10.18821/0016-9900-2019-98-1-105-117
- García-Moraleja A., Font G., Mañes J., Ferrer E. Simultaneous determination of mycotoxin in commercial coffee. Food Control, 2015, vol. 57, pp. 282–292. DOI: 10.1016/j.foodcont.2015.04.031
- Paterson R.R.M., Lima N., Taniwaki M.H. Coffee, mycotoxins and climate change. Food Research International, 2014, vol. 61, pp. 1–15. DOI: 10.1016/j.foodres.2014.03.037
- Bokhari F.M., Aly M.M. Evolution of traditional means of roasting and mycotoxins contaminated coffee beans in Saudi Arabia. Advances in Biological Research, 2009, vol. 3, no. 3–4, pp. 71–78.
- Oliveira G., da Silva D.M., Pereira R., Paiva L.C., Prado G., Batista L.R. Effect of different roasting levels and particle sizes on ochratoxin A concentration in coffee beans. Food Control, 2013, vol. 34, no. 2, pp. 651–656. DOI: 10.1016/j.foodcont.2013.06.014
- COMMISSION REGULATION (EC) No. 1881/2006 of 19 December 2006 setting maximum levels for certain contaminants in foodstuffs. Official Journal of the European Union, December 20, 2006. Available at: https://eur-lex.europa.eu/legal-content/EN/TXT/PDF/?uri=CELEX: 32006R1881&from=EN (01.11.2021).
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