Association between HSPA1B, S100B, and TNF genes polymorphisms and risks of chronic mercury poisoning

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Yu.I. Chernyak


East-Siberian Institute of Medical and Ecological Research, 12 a the 3rd micro-district, Angarsk, 665827, Russian Federation


We examined association between HSPA1B (+1267A/G, rs1061581), TNF-α (–308G/A, rs1800629), and S100B (C/T, rs9722) genes polymorphisms and chronic mercury poisoning (CMP).
PCR-RFLP analysis was used to examine a cohort consisting of 128 workers who were chronically exposed to mercury vapor; workers were distributed into two groups. The group 1 was made up of workers with long working experience who didn’t have CMP (n = 46), the group 2 included patients with long-term CMP period (n = 82). In addition, we estimated frequencies of rs1061581genotypes in 298 practically healthy men from regional sub-population (group 3).
HSPA1B (+1267A/G) polymorphic variant was established to have more frequent carriage of both minor G allele (р = 0.003) and a rare GG homozygote (р = 0.005) in the group 2 against the group 1. 23.2 % patients from the group 2 turned out to have GG genotype and CMP was diagnosed in 95 % people who had it. We didn’t detect any differences in genotypes distribution among people from the examined occupational cohort (groups 1 and 2) against the group 3. GG-HSP1AB (+1267A/G) homozygous genotype was shown to be associated with CMP risks (OR = 13.57, p < 0.0001, recessive model). Haplotype G–G (rs1061581–rs1800629) carriers were established to run 2.6 higher risks of CMP occurrence (р = 0.0098), and there was a significant linkage disequilibrium D' = 0.459 (р = 0.0004) between a pair of the abovementioned polymorphic loci. These data indicate that there is genetic interaction between HSPA1B (+1267A/G) and TNF-α (–308G/A) loci in the examined cohort.
Overall, these results indicate that carriers of GG-HSPA1B (+1267A/G) genotype run high predictive risks of CMP occurrence.

mercury, chronic exposure, chronic mercury poisoning, gene polymorphism, heat shock proteins 70, tumor necrosis factor, protein S100B, risk
Chernyak Yu.I. Association between HSPA1B, S100b, and TNF-α genes polymorphisms and risks of chronic mercury poisoning. Health Risk Analysis, 2021, no. 1, pp. 126–132. DOI: 10.21668/health.risk/2021.1.13.eng
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