Assessing sensitivity of campylobacter jejuni to anti-microbe effects to reduce risks of food prodycts contamination with campylobacteriosis agents

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N.R. Efimochkina, V.V. Stetsenko, Yu.M. Markova, L.P. Minaeva, I.B. Bykova, T.V. Pichugina, S.A. Sheveleva


Federal Research Center for Nutrition, Biotechnology and Food Safety, 2/14 Ust'inskiy proezd, Moscow, 109240, Russian Federation


We aimed to assess efficiency of various anti-microbe effects on pathogenic microflora; to do that, we performed com-parative study on how sensitive Campylobacter jejuni test strains were to ultraviolet radiation and biocides based on peracetic acid (PAA). PAA-based biocides suppressed significant numbers of Campylobacter; however, preset treating modes didn’t allow achieving complete inactivation of the test strains. Efficiency of effects produced by ultraviolet radiation on C.jejuni strains depended on exposure duration: 20-minute treatment of plates with broth bacterial suspensions caused a decrease in a number of viable cells which was equal to 1.5–2,0 logarithmic orders; 60-minute treatment resulted in a decrease in C.jejuni contents that amounted to less than 200 CFU/cm3.

Polypeptide antibiotics produced by lactic-acid bacteria (bacteriocins, nisin, etc.) have some useful properties and it makes them applicable for suppressing adverse microflora in food products manufacture. We applied an ‘associate growth” model to examine peculiarities related to C.jejuni growth in milk that, beside this pathogen, was simultaneously inoculated with mesophilic lactic-acid lactococci or thermophilic lactic-acid bacteria that were bacteriocins producers. Depending on quantity of introduced lactic-acid bacteria, such as Lactobacillusplantarum, Lactobacilluslactis and Lactococcuslactis, C.jejuni growth was substantially inhibited. We revealed a relationship between anti-bacterial activity and nature of inhibiting effects and concentrations of the above-mentioned lactic-acid microorganisms, temperature, an amount of time during mixed cultures were cultivated together, and properties of used strains which became the most apparent when lactobacteria were introduced in a dose equal to 108 CFU/cm3.

Our study on an ability of C.jejuni to survive under exposure to an adverse environment revealed that microbe populations had variable physiological properties, Campylobacter were greatly resistant, and it was difficult to make a relevant choice on efficient tools and procedures for anti-microbe treatment.

Campylobacter jejuni, in vitro model, biocides, anti-microbe effects, ultraviolet radiation, contamination, biofilms, lactic-acid bacteria
Efimochkina N.R., Stetsenko V.V., Markova Yu.M., Minaeva L.P., Bykova I.B., Pichugina T.V., Sheveleva S.A. Assessing sensitivity of campylobacter jejuni to anti-microbe effects to reduce risks of food prodycts contamination with campylobacteriosis agents. Health Risk Analysis, 2019, no. 4, pp. 139–147. DOI: 10.21668/health.risk/2019.4.15.eng
  1. Guerin M.T., Sir C., Sargeant J.M., Waddell L., O'Connor A.M., Wills R.W., Bailey R.H., Byrd J.A. The change in prevalence of Campylobacter on chicken cascasses during processing: A systematic review. Poult. Sci., 2010, vol. 89, no. 5, pp. 1070–1084. DOI: 10.3382/ps.2009-00213
  2. Yang H., Li Y., Johnson M.G. Survival and death of Salmonella typhimurium and Campylobacter jejuni in processing water and on chicken skin during poultry scalding and chilling. J. Food Prot, 2001, vol. 64, no. 6, pp. 770–776. DOI: 10.4315/0362-028x-64.6.770
  3. Vidal A.B., Davies R.H., Rodgers J.D., Ridley A., Clifton-Hadley F. Epidemiology and Control of Campylobacter in Modern Broiler Production. Campylobacter Ecology and Evolution. Norfolk, UK, Caister Academic Press Publ., 2014, vol. 1, pp. 287–231.
  4. Teh A.H., Lee S.M., Dykes G.A. Does Campylobacter jejuni form biofilms in food-related environment? Appl. Environ. Microbiol, 2014, vol. 80, no. 17, pp. 5154–5160. DOI: 10.1128/AEM.01493-14
  5. An Evaluation of EPA’s Proposed Guidelines for Carcinogen Risk Assessment Using Chloroform and Dichloroacetate as Case Studies. Report of an Expert Panel. International Life Sciences Institute, 1997. Available at:¬ro¬ace-tate-as-case-studies-workshop-report/ (20.07.2019).
  6. Christian M.S., York R.G., Hoberman A.M., Fisher L.C., Brown W.R. Oral (drinking water) two-generation reproduc-tive toxicity study of bromodichloromethane (BDCM) in rats. International Journal of Toxicology, 2002, vol. 21, no. 2, pp. 115–146. DOI: 10.1080/10915810252866097
  7. Hicks S.J., Rowbury R.J. Resistance of attached Escherichia coli to acrylic acid and its significance for the survival of plasmid-bearing organisms in water. Ann. Inst. Pasteur, 1987, vol. 138, no. 3, pp. 359–369. DOI: 10.1016/0769-2609(87)90124-4
  8. Scientific Opinion of the Panel on Biological Hazards on a request from DG SANCO on the assessment of the possible effect of the four antimicrobial treatment substances on the emergence of antimicrobial resistance. The EFSA Journal, 2008, no. 659, pp. 1–26.
  9. Mohyla P., Bilgili S.F., Oyarzabal O.A., Warf C.C., Kemp G.K. Application of Acidified Sodium Chlorite in the Drinking Water to Control Salmonella serotype Typhimurium and Campylobacter jejuni in Commercial Broilers. J. Appl. Poult Res, 2007, vol. 16, no. 1, pp. 45–51. DOI: 10.1093/japr/16.1.45
  10. Nikaido H. Multidrug resistance in bacteria. Annu Rev. Biochem, 2009, no. 78, pp. 119–146. DOI: 10.1146/an-nurev.biochem.78.082907.145923
  11. European Food Safety Authority and European Centre for Disease Prevention and Control. The European Union summary report on antimicrobial resistance in zoonotic and indicator bacteria from humans, animals and food in 2014. EFSA Journal, 2016, vol. 14, no. 2, 270 p. DOI: 10.2903/j.efsa.2018.5182
  12. Mavri A., Ribič U., Možina S.S. The Biocide and Antibiotic Resistance in Campylobacter jejuni and Campylobacter coli. Emerging and Traditional Technologies for Safe, Healthy and Quality Food, 2015, pp. 269–283. DOI: 10.1007/978-3-319-24040-4_15
  13. Christian M.S., York R.G., Hoberman A.M., Diener R.M., Fisher L.C., Gates G.A. Biodisposition of dibromoacetic acid (DBA) and bromodichloromethane (BDCM) administered to rats and rabbits in drinking water during range-finding repro-duction and developmental toxicity studies. International Journal of Toxicology, 2001, vol. 20, no. 4, pp. 239–253.
  14. Rao M.V. Acidified Sodium Chlorite (ASC). Chemical and Technical Assessment. Prepared for the 68th the Joint FAO/WHO Expert Committee on Food Additives. JECFA, 2007, 12 p. Available at: (20.07.2019).
  15. Oyarzabal O.A. Reduction of Campylobacter spp. by commercial antimicrobials applied during the processing of broiler chickens: a review from the United States perspective. J. Food Prot, 2005, vol. 68, pp. 1752–1760. DOI: 10.4315/0362-028x-68.8.1752
  16. The global view of campylobacteriosis: report of an expert consultation, Utrecht, Netherlands, 9–11 July 2012. World Health Organization, 2013, 69 p. Available at: (20.07.2019).
  17. Stetsenko V.V., Efimochkina N.R., Pichugina T.V. Osobennosti rosta i persistentsii Campylobacter jejuni v pishchevykh produktakh [Peculiarities of Campylobacter jejuni growth and persistence in food products]. Byulleten' eksperi-mental'noi biologii i meditsiny, 2018, vol. 166, no. 12, pp. 723–730 (in Russian).
  18. Efimochkina N.R., Stetsenko V.V., Bykova I.B., Polyanina A.S., Aleshkina A.S., Sheveleva S.A. Osobennosti kul'tivirovaniya i khraneniya bakterii roda Campylobacter v usloviyakh in vitro [Peculiarities of cultivating and storing Campylobacter bacteria in in vitro conditions]. Byulleten' eksperimental'noi biologii i meditsiny, 2017, vol. 164, no. 7, pp. 81–88 (in Russian).
  19. Efimochkina N.R., Bykova I.B., Korotkevich Yu.V., Markova Yu.M., Minaeva L.P., Sheveleva S.A. Study of tolerance of enterobacteria to chlorine-based biocides in experimental models using chromogenic indicator tests. Health Risk Analysis, 2015, no. 3, pp. 73–82 (in Russian). DOI: 10.21668/health.risk/2015.3.11.eng
  20. Efimochkina N.R., Bykova I.B., Markova Yu.M., Korotkevich Yu.V., Stetsenko V.V., Minaeva L.P., Sheveleva S.A. Formirovanie bioplenok pishchevymi patogenami i razrabotka na ikh osnove laboratornoi modeli in vitro dlya issledovaniya bakterii roda Campylobacter [Food pathogens forming biofilms and an in vitro laboratory model based on such films and applied to examine bacteria from Campylobacter family]. Byulleten' eksperimental'noi biologii i meditsiny, 2016, vol. 162, no. 10, pp. 470–475 (in Russian).

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