Genetically modified food products: peculiarities of genetic identification
G.F. Mukhammadiyeva1, D.O. Karimov1, O.V. Dolgikh2, A.V. Krivtsov2, A.A. Mazunina2
1Ufa Research Institute of Occupational Health and Human Ecology, 94 Stepan Kuvykin Str., Ufa, 450106, Russian Federation
2Federal Scientific Center for Medical and Preventive Health Risk Management Technologies, 82 Monastyrskaya Str., Perm, 614045, Russian Federation
Our research goal was to perform a genetic analysis of food stuffs produced in Russia in order to determine whether ge-netically modified components, predominantly soya, occurred in them. We also set a task to draw up an optimal list of genetic modifiers for sausages and soya products; this list was to be applied as a tool for monitoring of undeclared GMO and for providing biological safety of food stuffs. We applied polymerase chain reaction in real-time mode to analyze certain food prod-ucts (sausages and soya products); the analysis was to reveal genetically modified organisms. The task was to identify the fol-lowing GMO genes: promoters (p35SCaMV, P-SSuAra, Ubi1, ract1, hsp70, TA29 tobacco promoter), terminators (nos3, T-E9, T-g7, T-OCS), reporter genes (nptll, qHptFP308, bar, pat_10-P), bio-pesticides Bacillus Thuringensis (Bt) or Cry-toxins (Cry1Ab/Ac), reporter gene of β-glucuronidase (GUS-gene). Analysis of some sausage samples allowed us to identify the following GMO genes: Cry1Ab/Ac, P-FMV, P-nos, bar, gus_9-P, Т-nos3, nptii, P-TA29, T-E9, T-g7, T-OCS. The research performed on food products revealed GMO in 56 % of the analyzed sausage samples. Genetic modification of the analyzed food samples had its peculiarities; a set of identified genes that included promoter genes P-FMV, terminators (nos3, T-g7, T-OCS), Cry1Ab/Ac endo-toxin, and a reporter of GMO bar was one of them. We recommend to use the following candidate genes for GMO contents in food products: Cry1Ab/Ac, P-FMV, P-nos, bar, gus_9-P, Т-nos3, nptii, P-TA29, T-E9, T-g7, T-OCS. They all are evidence that genetic modifications took place and they all can be applied as marker genes for control over food products safety as per GMO contents criterion.
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