Separate quontitative determination of organic and non-organic arsenic in sea products

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614.3: 543.001.6:546

U.S. Kruglyakova, O.V. Bagryantseva, A.D. Evstratova, A.D. Malinkin, I.V. Gmoshinskii, S.A. Khotimchenko


Federal Research Center for Nutrition, Biotechnology and Food Safety, 2/14 Ust'inskiy lane, Moscow, 109240, Russian Federation


The performed research is truly vital, as As (arsenic) concentration in food products is now of great interest. The US ATSDR and EPA enlist As among the most toxic substances which are dangerous for human health.

We suggest a procedure for separate quantitative mass fraction determination for organic (oAs) and non-organic (iAs) arsenic compounds in sea products with solid phase extraction (SPE) application combined with atomic adsorption spectrometry. Samples were prepared according to the following procedure: liquid extraction phase with simultaneous As (III) oxidation into As (IV) with hydrogen peroxide and As (V) extraction into a 0.055 M liquid phase with hydrochloric acid. Arsenic organic and non-organic compounds were separated via solid phase extraction with Strata SAX cartridges (Sorbent Lot Number: S208-0058). To quantitatively assess the obtained samples, we applied atomic-adsorption techniques for As determination with "KVANT-2A-GRG" spectrometer according to the State Standard 51766-2001. We revealed that common As concentration didn't conform to fixed standards in 8 out of 17 analyzed samples (2 shrimps, 1 crab, 1 fish, and 4 seaweeds). However, iAs concen-tration was significantly lower than oAs concentration in all the samples. 6 out of 17 analyzed samples didn't contain any iAs within detection limits (0.1 mg/kg), and apparently all the As concentration occurred due to its organic compounds. The suggested procedure for separate oAs and iAs detection is relatively simple in terms of devices applied in it, and quite cheap, as SPE cartridges needed to perform it can be re-used after re-conditioning. This procedure, after a proper metrological validation, can be implemented in most laboratories which are certified to examine chemical safety of food products.

arsenic, non-organic form, solid phase extraction, atomic-adsorption spectrometry, risk assessment
Kruglyakova U.S., Bagryantseva O.V., Evstratova A.D., Malinkin A.D., Gmoshinskii I.V., Khotimchenko S.A. Separate quontitative determination of organic and non-organic arsenic in sea products. Health Risk Analysis, 2018, no. 2, pp. 112–118. DOI: 10.21668/health.risk/2018.2.13.eng
  1. Gomez-Caminero A., Howe P., Hughes M., Kenyon E., Lewis D.R. [et al.]. Arsenic and arsenic compounds. Geneva: World Health Organization, 2001, 521 p.
  2. Dietary exposure to inorganic arsenic in the European population. EFSA Journal, 2014, vol. 12, no. 3, pp. 3597.
  3. Environmental Health and Medicine Education. Arsenic Toxicity. Cover Page. Agency for Toxic Substances and Disease Registry. Available at: (16.03.2018).
  4. Hughes M.F., Beck B.D., Chen Yu., Lewis A.S., Thomas D.J. Arsenic exposure and toxicology: a historical perspective. Toxicological Sciences, 2011, vol. 123, no. 2, pp. 305–332.
  5. List of classifications, Volumes 1–121. World Health Organization: IARC Monographs on the Evaluation of Carcinogenic Risks to Humans. Available at: (16.03.2018).
  6. Schoof R.A., Yost L.J., Eickhoff J., Crecelius E.A., Cragin D.W., Meacher D.M., Menzel D.B. A market basket survey of inorganic arsenic in food. Food Chem. Toxicol., 1999, vol. 37, pp. 839–846.
  7. Jackson B.P., Bertsch P.M. Determination of arsenic speciation in poultry wastes by ic-icp-ms. Environ. Sci. Technol., 2001, vol. 35, pp. 4868–4873.
  8. Stolz J.F., Perera E., Kilonzo B., Kail B., Crable B., Fisher E., Ranganathan M., Wormer L., Basu P. Biotransformation of 3-nitro-4-hydroxybenzene arsonic acid (roxarsone) and release of inorganic arsenic by clostridium species. Environ. Sci. Technol., 2007, vol. 41, pp. 818–823.
  9. Feldmann J., Krupp E.M. Critical review or scientific opinion paper: Arsenosugars-a class of benign arsenic species or justification for developing partly speciated arsenic fractionation in foodstuffs. Anal. Bioanal. Chem., 2011, vol. 399, pp. 1735–1741.
  10. Arsenic, Metals, Fibres, and Dusts. IARC Monographs on the Evaluation of Carcinogenic Risks to Humans Volume 100C. World Health Organization: International Agency for Research on Cancer. Available at: (16.03.2018).
  11. Sabirova K.M., Kislitsina L.V., Kiku P.F. Otsenka riska dlya zdorov'ya naseleniya Primorskogo kraya ot vozdeistviya mysh'yaka v produktakh pitaniya [Risk assessment for health of population of Primorsky krai from exposure to arsenic in foods]. Zdorov'e. Meditsinskaya ekologiya. Nauka, 2017, vol. 70, no. 3, pp. 139–142 (in Russian).
  12. Sabirova K.M., Kislitsyna L.V., Kiku P.F. Otsenka riska dlya zdorov'ya naseleniya ot voz-deistviya mysh'yaka [Assessment of risk for health of the population from the effects of arsenic]. Zdorov'e naseleniya i sreda obitaniya, 2017, no. 9 (294), pp. 47–51 (in Russian).
  13. Valeullina N.N., Ural'shin A.G., Brylina N.A., Grechko G.Sh., Beketov A.L., Nikiforova E.V., Makhanova I.I. Soderzhanie mysh'yaka v khlebnykh produktakh i otsenka riska dlya zdorov'ya naseleniya pri ikh potreblenii [Arsenic contents in bakery and assessment of population health risk related to such products]. Aktual'nye problemy bezopasnosti i analiza riska zdorov'yu naseleniya pri vozdeistvii faktorov sredy obitaniya: Materialy VII Vserossiiskoi nauchno-prakticheskoi konferentsii s mezhdunarodnym uchastiem. In: A.Yu. Popova, N.V. Zaitseva eds. Perm, 2016, vol. 2, pp. 18–21 (in Russian).
  14. Shumakova A.A., Povarova N.M., Rezaeva D.M. Soderzhanie nekotorykh toksichnykh elemen-tov v krupakh i zerne [Concentrations of some toxicants in cereals and grains]. Voprosy pitaniya, 2016, vol. 85, no. S2, pp. 39 (in Russian).
  15. Chuprakova A.M., Rebezov M.B. Analiz rezul'tatov monitoringa prob myasnykh i rybnykh produktov na soderzhanie tyazhelykh metallov [The analysis of monitoring results of samples of meat and fish on the content of heavy metals]. Vestnik Yuzhno-Ural'skogo gosudarstvennogo universiteta. Seriya: Ekonomika i menedzhment, 2015, vol. 9, no. 2, pp. 194–201 (in Russian).

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