Assessment of the airborne phthalate exposure on lung function of preschool and primary school children

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T.S. Ulanova1,2, T.D. Karnazhitskaya1, E.O. Zavernenkova1


1 FBSI “Federal Scientific Center for Medical and Preventive Health Risk Management Technologies”, 82 Monastyrskaya St., Perm, Russian Federation, 614045
2 FBSEI HPE “Perm National Research Polytechnic University”, Komsomolsky Av. 29, Perm, Russian Federation, 614990


The results of the studies of phthalates’ content in the indoor and outdoor air on the territory of pre-school and school educational institutions, the definition of phthalates metabolites in the urine and parameters of lung function of children attending these institutions are presented in the work. The HPLC/MS method detected the presence of 4 phthalates in 7 analyzed rooms and the air of pre-school and school facilities in the range 0.0002–0.00116 mg/m 3 concentrations. The method of HPLC/MS/TFE metabolites detected the presence of phthalate (monometylphtalate, monobutylphtalate, monohexylilphtalate) in the urine of children in the concentration range of 0.00039–0.012 mg/m 3 . Analysis of dependencies of "the concentration of phthalate metabolites in the urine – the parameters of pulmonary function" revealed a statistically significant correlation coefficients (p < 0.05) of directly proportional dependency of Gensler index (FEV1/SVC) and instantaneous space velocity (MEF 25, 50, 75) on the concentration of monophtalates in urine of the girls. A statistically significant correlation of coefficients (p < 0.05) inversely proportional to the forced vital capacity (FVC), forced expiratory volume in the first second (FEV1) and the instantaneous volume velocity after exhaling 50 % (MEF 50) from monophtalates’ concentration in the boys’ urine.

phthalates monometylphtalate, monobutylphtalate, monobenzylphtalate, mono-2-ethylhexylphthalate, high performance liquid chromatography / mass spectrometry, atmospheric air, lung function parameters
Ulanova T.S., Karnazhitskaya T.D., Zavernenkova E.O. Assessment of the airborne phthalate exposure on lung function of preschool and primary school children. Health Risk Analysis, 2016, no. 1, pp. 50-58. DOI: 10.21668/health.risk/2016.1.06.eng
  1. Glanc S. Mediko-biologicheskaja statistika [Biomedical Statistics]. Moscow: Praktika, 1999, 459 p. (in Russian).
  2. May I.V., Khoroshavin V.A., Evdoshenko V.S. Algoritm i metody sanitarno-jepidemiologicheskogo rassledovanija narushenij prav grazhdan na blagoprijatnuju okruzhajushhuju sredu obitanija s jetapom ocenki riska dlja zdorov'ja [Algorithms and methods of sanitary-epidemiological investigation of civil rights violations on a favorable habitats with step of health risk assessment]. Zdorov'e naselenija i sreda obitanija, 2010, no. 11, pp. 28–30. (in Russian).
  3. Majstrenko V.N., Kljuev N.A. Jekologo-analiticheskij monitoring stojkih organicheskih zagrjaznitelej [Ecological and analytical monitoring of persistent organic pollutants]. BINOM, Laboratorija znanij, Moscow, 2004, 328 p. (in Russian).
  4. Zaitseva N.V., Ulanova T.S.. Nurislamova T.V., Karnazhickaya T.D., Plahova L.V., Suetina G.N. Metodi-cheskie podhody i kriterii ocenki pri opredelenii himicheskih soedinenij v biosredah [Methodical approaches and criteria in determining the chemical compounds in biological milieus]. Materialy Plenuma Laboratornogo Soveta gosudarstvennoj sanitarno-jepidemiologicheskoj sluzhby Rossijskoj Federacii, Moscow, 2004, pp. 59–64. (in Russian).
  5. Onishhenko G.G., Zaitseva N.V., Ulanova T.S. Kontrol' soderzhanija himicheskih soedinenij i jelementov v biologicheskih sredah [Control of chemical elements and compounds in biological milieus]. Perm': Izd-vo «Knizhnyj format», 2011, 520 p. (in Russian).
  6. Ulanova T.S. Sistema himiko-analiticheskoj podderzhki sanitarno-gigienicheskih issledovanij (mediko-biologicheskie aspekty) [The system of chemical-analytical support of sanitary research (medical and biological aspects)]. Gigienicheskie i mediko-profilakticheskie tehnologii upravlenija riskami zdorov'ju naselenija v promyshlenno razvityh regionah: materialy nauch.-prakt. konf. s mezhdunar. Uchast. Perm', 2010, pp. 62–67. (in Russian).
  7. Gray L.E., Jr, Wolf C., Lambright C., Mann P., Price M., Cooper R.L., Ostby J. Administration of potentially antiandrogenic pesticides (procymidone, linuron, iprodione, chlozolinate, p,p'-DDE, and ketoconazole) and toxic substances (dibutyl and diethylhexyl phthalate, PCB 169, and ethane dimethane sulphonate) during sexual differentiation produces diverse profiles of reproductive malformations in the male rat. Toxicol Ind Health, 1999, no. 15 (1–2), pp. 94–118.
  8. Blount B., Silva M., Caudill S., Needham L., Pirkle J., Sampson E., Lucier G., Jackson R., Brock J. Levels of seven urinary phthalate metabolites in a human reference population. Environ. Health. Perspect, 2000, no. 108 (10), pp. 979–982.
  9. Hoppin, J.A, Ulmer, R. & London, S.J. (2004) Phthalate Exposure and Pulmonary Function. Environmental Health Perspectives, 2004, no. 112 (5), pp. 571–574
  10. Jaakkola J.J., Verkasalo P.K., Jaakkola N. Plastic Wall Materials in the Home and Respiratory Health in Young Children. Am. Journal of Public Health, 2000, Vol. 90, no. 5, pp. 797– 799.
  11. Mylchreest E, Cattley RC, Foster PM. Male reproductive tract malformations in rats following gestational and lactational exposure to Di (n-butyl) phthalate: an antiandrogenic mechanism?. Toxicol Sci, 1998, no. 43, pp. 47–60.
  12. Rudel R., Camann D., Spengler J., Korn L., Brody J. Phthalates, Alkylphenols, pesticides, polybrominated diphenyl ethers, and other endocrine-disrupting compounds in indoor air and dust. Environmental Science and Technology, 2003, no. 37 (20), pp. 4543–4553.
  13. Substance Monograph: Di (2-ethylhexyl) phthalate (DEHP). Opinion of the Human Biomonitoring Com-mission of the German Federal Environmental Agency, Published in: Bundesgesundheitsblatt – Gesundheitsfor-schung – Gesundheitsschutz, 2005, no. 48 (6), pp. 706–722.
  14. Ponsonby A.L., Dwyer T., Kemp A., Cochrane J., Couper D., Carmichael A. Synthetic Bedding and Wheeze in Childhood. Epidemiology, 2003, no. 14, pp. 37–44.
  15. Toxicological Profile for Di (2-ethylhexyl) Phthalate. U.S. Department of Health and Human Services, Public Health Service, Agency for Toxic Substances and Disease Registry, 1993, 291 p.
  16. Toxicological Profile for DiethylPhthalate. U.S. Department of Health and Human Services, Public Health Service, Agency for Toxic Substances and Disease Registry, 1993, 125 p.
  17. Toxicological Profile for Di-n-butylPhthalate. U.S. Department of Health and Human Services, Public Health Service, Agency for Toxic Substances and Disease Registry, 1990, 184 с.

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