Development of methodical approach to the identification of the features of the genetic polymorphisms and gene expression in children under influence of chemical environmental factors on the example of strontium

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DOI: 
10.21668/health.risk/2016.1.04.eng
Authors: 

O.V. Dolgikh1,2,3, N.V. Zaitseva1,2,3, A.V. Krivtsov1, K.G. Starkova1, D.G. Dianova1, O.A. Bubnov1,3, E.A. Otavina1, N.V. Bezruchenko1,3

Organization: 

1 FBSI “Federal Scientific Center for Medical and Preventive Health Risk Management Technologies”, Russian Federation, Perm, 82 Monastyrskaya St., 614045
2 FBSEI HPE “Perm National Research Polytechnic University”, Russian Federation, Perm Komsomolsky Av., 29, 614990
3 FBSEI HPE “Perm State National Research University”, Russian Federation, Perm, 15 Bukireva St., 614990

Abstract: 

Methodological approaches evaluating the features of genetic polymorphism associated with exposure to chemical etiology factors for the identification of genetic susceptibility markers were developed. The technologies, methodological aspects of the use of polymerase chain reaction, DNA sequencing fragments, studies of spontaneous and induced strontium expression of candidate genes that help identify changes in the genome and transcriptome in order to identify early disorders of adaptation processes in chronic environmental burden to prove the injury and assessment of individual exposure risk chemical factors were suggested.

Keywords: 
sensitivity markers, genetic polymorphisms, gene expression, sequencing, strontium
Dolgikh O.V., Zaitseva N.V., Krivtsov A.V., Starkova K.G., Dianova D.G., Bubnov O.A., Otavina E.A., Bezruchenko E.A. Development of methodical approach to the identification of the features of the genetic polymorphisms and gene expression in children under influence of chemical environmental factors on the example of strontium. Health Risk Analysis, 2016, no. 1, pp. 34-41. DOI: 10.21668/health.risk/2016.1.04.eng
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