Neurotransmitter system of immune regulation as a marker of immunological disorders in pupils in the conditions of increased entry of strontium with drinking water

View or download the full article: 

О.V. Dolgikh1,2, А.V. Krivtsov1, К.G. Starkova1, V.А. Luchnikova1, О.А. Bubnova1,2, D.G. Dianova1, N.V. Bezruchenko1,2, N.A. Vdovina1


1 FBSI “Federal Scientific Center for Medical and Preventive Health Risk Management Technologies”, Russian Federation, Perm, 82 Monastyrskaya St., 614045
1 FBSEI HPE “Perm State National Research University”, Russian Federation, Perm, 15 Bukireva St., 614990


The evaluation of immunological markers in schoolchildren exposed to strontium is performed. It is shown that under the conditions of increased administration of strontium with drinking water the indication of spontaneous and induced levels of neurotransmitters in vitro allows to detect early functional disorders of the immune system. It was found that the following markers of specific hypersensitivity and mediators of intercellular immune regulation (IgG specific to strontium, cytokines IL-6, IL-10, IL-12, IL-17, α-TNF, GM-CSF, spontaneous and specifically stimulated, RANKL, OPG( may be proposed for the identification of health risk as early markers of immune disorders in school children living in areas of strontium geochemical provinces.

strontium, cytokines, markers
Dolgikh О.V. , Krivtsov А.V., Starkova К.G., Luchnikova V.А., Bubnova О.А., Dianova D.G., Bezruchenko N.V., Vdovina N.A. Neurotransmitter system of immune regulation as a marker of immunological disorders in pupils in the conditions of increased entry of strontium with drinking water. Health Risk Analysis, 2015, no. 3, pp. 61-67. DOI: 10.21668/health.risk/2015.3.09.eng
  1. Vengerovskiy A.I., Hlusov I.A., Nechaev K.A. Molekuljarnye mehanizmy dejstvija bisfosfonatov i stronci-ja ranelata [Molecular mechanisms of bisphosphonate and strontium ranelate action]. Jekcpep. i klin. fapmak, 2014, no. 77 (9), pp. 43–46.
  2. Dolgikh O.V., Predeina R.A., Dianova D.G. Jeksperimental'naja ocenka vlijanija fenolov na immunoregul-jaciju ex vivo [Experimental evaluation of phenol effect on ex vivo immunoregulation]. Analiz riska zdorov'ju, 2014, no. 1, pp. 73–81.
  3. Dolgikh O.V., Zaitseva N. V., Dianova D.G., Lyhina T.S., Krivtsov A.V., Gugovich A.M. Osobennosti limfo-citarno-kletochnogo zvena u detej, prozhivajushhih na tehnogenno-nagruzhennyh territorijah [Peculiarities of lymphocyte cell level in children living in technologically laden areas]. Biol. membrany, 2012, no. 29 (5), pp. 349–353.
  4. Krzystyniak K. [et. al.] Approaches to the evaluation of chemical-induced immunotoxicity. Environ Health Perspect., 1995, vol. 103, suppl 9, pp. 17–22.
  5. Caverzasio J., Thouverey C. Activation of FGF receptors is a new mechanism by which strontium ranelate induces osteoblastic cell growth. Cell. Physiol. Biochem., 2011, no. 27 (3–4), pp. 243–250.
  6. Newby C.S. [et. al.] Cytokine release and cytotoxicity in human keratinocytes and fibroblasts induced by phenols and sodium dodecyl sulfate. Journal of Investigative Dermatology, 2000, vol. 115, pp. 292–298.
  7. Fromigué О., Haÿ Е., Barbara А. Calcium sensing receptor-dependent and receptor-independent activation of osteoblast replication and survival by strontium ranelate. JCMM, 2009, no. 13 (8B), pp. 2189–2199.
  8. Yang F., Yang D., Tu J., Zheng Q., Cai L., Wang L. 2011. Strontium enhances osteogenic differentiation of mesenchymal stem cells and in vivo bone formation by activating Wnt/catenin signaling. Stem cells. 2011, doi: 10.1002/stem.646.
  9. Caudrillier A., Hurtel-Lemaire A.-S., Wattel A., Cournarie F., Godin C., Petit L., Petit J.-P., Terwilliger E., Kamel S., Brown E. M., Mentaverri R., Brazier M. Strontium ranelate decreases RANKL-induced osteoclastic dif-ferentiation in vitro: involvement of the calcium sensing receptor. Mol. pharmacol, 2010, no. 4, pp. 569–576.
  10. Los M., Maddika S., Erb B., SchulzeOsthoff K. Switching Akt: From survival signaling to deadly response. BioEssays, 2009, no. 31 (5), pp. 492–495.
  11. Hurtel-Lemaire A.S., Mentaverri R., Caudrillier A., Cournarie F., Wattel A., Kamel S., Terwilliger E.F., Brown E.M., Brazier M. The calcium-sensing receptor is involved in strontium ranelate-induced osteoclast apopto-sis. New insights into the associated signaling pathways. JBC, 2009, no. 284, pp. 575–584.
  12. Huang C., Li L., Yu X., Gu Z., Zhang X. The inhibitory effect of strontium-doped calcium polyphosphate particles on cytokines from macrophages and osteoblasts leading to aseptic loosening in vitro. Biomed. mater, 2014, no. 9 (2). doi: 10.1088/1748-6041/9/2/025010.
  13. Tucci P. Caloric restriction: is mammalian life extension linked to p53? AGING, 2012, no. 8, pp. 525–534.
  14. Li V.S., Ng S.S., Boersema P.J., Low T.Y., Karthaus W.R., Gerlach J.P., Mohammed S., Heck A.J., Maurice M.M., Mahmoudi T., Clevers H. Wnt signaling through inhibition of β-catenin degradation in an intact axin1 com-plex. Cel., 2012, vol. 149, pp. 1245–1256.
  15. Yurchenko M., Shlapatska L.M., Sidorenko S.P. The multilevel regulation of CD95 signaling outcome. Exp. oncol., 2012, no. 34 (3), pp. 200–2011.
  16. Zaytseva N.V., Dianova D.G., Dolgykh O.V. 2014. Еffects of cellular immunity in conditions of surplus supply of strontium with consumed water. EJNH, no. 1, pp. 7–8.

You are here