Comparison of toxicity of aqueous suspension of nano-and microfine silica in subchronic experiment

View or download the full article: 
615.9, 614.7

M.A. Zemlyanova1, V.N. Zvezdin1, A.A. Dovbysh1, T.I. Akafyeva1,2


1 FBSI “Federal Scientific Center for Medical and Preventive Health Risk Management Technologies”, 82, Monastyrskaya St., Perm, 614045, Russia,
2 FSBEI HPE “Perm State National Research University”, 15, Bukireva St., Perm, 614990, Russia


In subchronic experiment on Wistar’s white male rats the negative effects have been studied and the comparative assessment of toxicity of an aqueous suspension of nano- and microfine silicon dioxide has been carried out. It was found that by intragastric administration for 90 days of the dose of 14.6 mg/kg, corresponding to the predicted threshold dose (LOAEL), the aqueous suspension of nanosized silicon dioxide causes immune irritating, cytolytic and inflammatory effects, verified by the deviation of hematologic parameters and morphological changes in tissues bodies / or target systems (liver, stomach, jejunum and colon, immune system). Micro-sized silica causes predominantly an inflammatory effect in the mucosa of stomach, jejunum and colon.

silica nanoparticles, microparticles, negative effects, subchronic toxicity, intragastric administration, experimental studies
Zemlyanova M.A., Zvezdin V.N., Dovbysh A.A., Akafyeva T.I. Comparison of toxicity of aqueous suspension of nano-and microfine silica in subchronic experiment. Health Risk Analysis, 2014, no. 1, pp.74-82
  1. Bljuger A.F., Majore A.Ja. Problemy perekisnogo okislenija lipidov v gepatologii [Problems of lipid per-oxidation in hepatology]. Uspehi gepatologii, 1978, no. 7, pp. 22–54.
  2. Glanc S. Mediko-biologicheskaja statistika [Biomedical statistics]. Ed. N.E. Buzikashvili i soavt. Moscow: Praktika, 1998. 459 p.
  3. Nanoporoshki: opisanie i ob#emy proizvodstva: Marketingovyj otchet Is-sledovatel'skoj kompanii «Abercade» [Nanopowders: description and production: Market Report of the Research company «Abercade». 2008]. 2008. Avaible at: URL: http: //
  4. Zajceva N.V., Zemljanova M.A., Zvezdin V.N., Saenko E.V. Toksikologo-gigienicheskaja ocenka be-zopasnosti vodnoj suspenzii nanodispersnogo dioksida kremnija, sintezirovannogo metodom zhidkokristal-licheskogo templatirovanija [Toxicological and hygienic evaluation of the safety of an aqueous suspension of nanopowder silica synthesized by the liquid crystal templating]. Analiz riska zdorov'ju, 2013, no. 1, pp. 65–72.
  5. Guide for the care and use of laboratory animals. Eighth Edition. Committee for the Update of the Guide for the Care and Use of Laboratory Animals; Institute for Laboratory Animal Research (ILAR); Division on Earth and Life Studies (DELS); National Research Council of the national academies. Washington: The national acad-emies press, 2011. 246 p.
  6. Meynen V., Cool P., Vansant E.F. Verified syntheses of mesoporous materials. Microporous and mesopor-ous materials, 2009, vol. 25, pp. 170–223.
  7. Donaldson K. Stone V., Tran C.K. et al. Nanotoxicology (editorial). Occupat. and Environm. Med., 2004, vol. 61, pp. 727–728.
  8. Ghiazza M., Carella E., Tomatis M., Corazzari M., Cristina M. Predictive tests to evaluate oxidative poten-tial engineered nanomaterials. Nanotechnology and nanotoxicology, Grenoble, 13–15 November 2012.
  9. Bastus N.G., Casals E., Socono V.S., Puntes V. Reactivity of engineered inorganic nanoparticles and car-bon nanostructures in biological media. Nanotoxicology, 2008, vol. 2, pp. 99–112.
  10. Nozawa K. Gailhanou H., Raison L., Panizza P. Smart Control of Monodisperse Stolber Silica Particles: Effect of Reactant Addition Rate on Growth Process. Langmuir, 2005, vol. 21, pp. 1516–1523.
  11. Vallet-Regi M., Balas F., Arcos D. Mesoporous Materials for Drug Delivery. Angew. Chem. Int. Ed., 2007, vol. 46, pp. 7548–7558.
  12. World Nanotechnology: Innovation and Technology Trends. Nanotechnology Industry Market Research Reports. 2012. Avaible at: http: // 319_1.pdf.

You are here